Development of a two-step enrichment procedure for recovery of Yersinia enterocolitica from food
نویسندگان
چکیده
منابع مشابه
Comparison of Enrichment Schemes for the Isolation of Yersinia Enterocolitica
Discussion: Considering the effect of sample weight on the detection of Salmonella bacteria (Davies et al., 2000), we cannot directly compare the three enrichment media used herein. However, we suggest the use of M1 whenever possible to obtain large amounts of fecal material (10g). Furthermore, the use of direct enrichment of swine feces in SC is discouraged. The coupling of RV to PCR was signi...
متن کاملClinical isolation of Yersinia enterocolitica: cold temperature enrichment.
A cold-temperature enrichment procedure was used to isolate Yersinia enterocolitica serotype 6 from a clinical stool specimen. The use of conventional laboratory media and enrichment procedures failed to isolate this organism.
متن کاملModified enrichment broth for isolation of Yersinia enterocolitica from nonfood sources.
Sorbitol-bile salts broth was modified by the addition of 0.5% peptone for the enrichment of Yersinia enterocolitica in nonfood samples. A quantitative comparison was made of the growth of Y. enterocolitica in sorbitol-bile salts broth and peptone-sorbitol-bile salts broth at 22 degrees C. Incubation for 48 h resulted in an average 7.79 log10 increase in a viable cell number for six serotypes w...
متن کاملNew enrichment method for isolation of pathogenic Yersinia enterocolitica serogroup O:3 from pork.
A new enrichment medium for the recovery of pathogenic Yersinia enterocolitica serogroup O:3 from naturally infected meat products based on three selective agents, Irgasan, ticarcillin, and potassium chlorate (ITC), was compared with several other one- or two-step enrichments. Y. enterocolitica serogroup O:3 was recovered from 96.5% of 29 pork tongues, 24% of 50 ground pork samples, 16% of 25 m...
متن کاملمیزان شیوع گونه های یرسینیا در گوشت قرمز و مرغ عرضه شده در جنوب تهران
Background and Aim: Yersinia is an important water- and food-borne bacterium causing gastroenteritis in humans. Material and Methods: From December 2002 to July 2003, a total of 250 samples -including 158 meat samples and 92 chicken samples- were taken from butcheries and poultry shops operating under the supervision of Tehran University of Medical Sciences. We used a two-step enrichment proce...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1982
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.43.1.14-27.1982